rabbit polyclonal adrenergic receptor antibodies β2  (Fisher Scientific)

Journal: Scientific Reports

Article Title: β2-adrenergic signal transduction plays a detrimental role in subchondral bone loss of temporomandibular joint in osteoarthritis

doi: 10.1038/srep12593

Figure Lengend Snippet: A, B : Norepinephrine (NE) levels in serum ( A ) and condylar subchondral bone ( B ) over the three time-points (2, 4 and 8 weeks) examined. C : control rats; E: experimental rats. Levels of significance: *P < 0.05, **P < 0.01. C : Immunofluorescent staining and quantification of the tyrosine hydroxylase (TH) positive sympathetic nerve fibers in the condylar subchondral bone of 4-week control and experimental groups. The dash line indicates the interface between cartilage ( C ) and subchondral bone ( B ). Arrows indicate TH-positive sympathetic nerve fibers (red color). The blue color indicates cell nuclei stained by DAPI. D : Real-time PCR analysis of the mRNA expression of β-adrenergic receptors (β-ARs) in the condylar subchondral bone of 4-week control and experimental groups. E : Immunohistochemical staining and quantification of the β2-AR (Adrb2) positive cells in the condylar subchondral bone of 4-week control and experimental groups. Levels of significance for all charts: *P < 0.05, **P < 0.01.

Article Snippet: The primary antibodies were rabbit monoclonal tyrosine hydroxylase (TH; 1:100; ab75875, Abcam, Cambridge, MA, USA), rabbit polyclonal β2-adrenergic receptor (Adrb2; 1:100, ab137494; Abcam) and osteocalcin (1:100, sc-30045, Santa Cruz Biotechnology, Inc., Dallas, Texas, USA).

Techniques: Control, Staining, Real-time Polymerase Chain Reaction, Expressing, Immunohistochemical staining

Journal: PLoS ONE

Article Title: Standard Sub-Thermoneutral Caging Temperature Influences Radiosensitivity of Hematopoietic Stem and Progenitor Cells

doi: 10.1371/journal.pone.0120078

Figure Lengend Snippet: A: Average concentration of norepinephrine in plasma serum (measured by ELISA) in from ST (black bar) and TT (gray bar) mice (n = 5). B: Average percentage of active caspase-3+ LSK cells in ST mice treated with saline vehicle or propranolol versus TT mice treated with saline vehicle or propranolol 3 days after 300 cGy (left) or 600 cGy (right) TBI (n = 5). C: Percentage of active caspase-3+ LSK cells 2 days after irradiation with 300 cGy in vitro (n = 2). Cells were cultured for 48 hours with vehicle isoproterenol, or propranolol prior to irradiation. D: Average MFI of β2-adrenergic receptor on LSK cells from ST and TT mice (n = 2). For panels A-D, data are presented as mean ± SEM and p-values calculated using Student’s t test.

Article Snippet: After permeabilization, cells were stained with rabbit polyclonal anti-β2 adrenergic receptor antibody (Thermo Scientific, PA5-19649) followed by PE-conjugated goat anti-rabbit IgG.

Techniques: Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Saline, Irradiation, In Vitro, Cell Culture